streetsfinest:

zar-aa:

:)

Streets Finest

(anpan-and-fashionから)

Sebastian Stan and his Bucky feelings (◡‿◡✿)

(krescentmoonから)

fuckyeahpixivranking:

おめヽ(=´▽`=)ノ」/「anco@ティアT61b

(rollingbarrelから)

akunohomu:

Artist: かざめ

"骸音シーエ" by -匙-

*Permission was given from the artist to upload. Do not distribute.

akunohomu:

Artist: 自作ぎゃてコラ保管用アカウント

artsfantasia:

lazy dragon by Vuk Kostic

(A World of Fantasy)

ananthymous:

tally-art:

New Home Is Where The Internet Is: the case of the cleaning couchsurfer

I’ve always been really into Tally’s art, but lately every new drawing of hers has something new to love! Go Tally go!

(xantchawalkerから)

xantchawalker:

cupcakeplexi:

Story of my life

This is my anime so hard. Love Princess Jellyfish.

neurosciencestuff:

Rapid whole-brain imaging with single cell resolution

A major challenge of systems biology is understanding how phenomena at the cellular scale correlate with activity at the organism level. A concerted effort has been made especially in the brain, as scientists are aiming to clarify how neural activity is translated into consciousness and other complex brain activities. One example of the technologies needed is whole-brain imaging at single-cell resolution. This imaging normally involves preparing a highly transparent sample that minimizes light scattering and then imaging neurons tagged with fluorescent probes at different slices to produce a 3D representation. However, limitations in current methods prevent comprehensive study of the relationship. A new high-throughput method, CUBIC (Clear, Unobstructed Brain Imaging Cocktails and Computational Analysis), published in Cell, is a great leap forward, as it offers unprecedented rapid whole-brain imaging at single cell resolution and a simple protocol to clear and transparentize the brain sample based on the use of aminoalcohols.

In combination with light sheet fluorescence microscopy, CUBIC was tested for rapid imaging of a number of mammalian systems, such as mouse and primate, showing its scalability for brains of different size. Additionally, it was used to acquire new spatial-temporal details of gene expression patterns in the hypothalamic circadian rhythm center. Moreover, by combining images taken from opposite directions, CUBIC enables whole brain imaging and direct comparison of brains in different environmental conditions.

CUBIC overcomes a number of obstacles compared with previous methods. One is the clearing and transparency protocol, which involves serially immersing fixed tissues into just two reagents for a relatively short time. Second, CUBIC is compatible with many fluorescent probes because of low quenching, which allows for probes with longer wavelengths and reduces concern for scattering when whole brain imaging while at the same time inviting multi-color imaging. Finally, it is highly reproducible and scalable. While other methods have achieved some of these qualities, CUBIC is the first to realize all.

CUBIC provides information on previously unattainable 3D gene expression profiles and neural networks at the systems level. Because of its rapid and high-throughput imaging, CUBIC offers extraordinary opportunity to analyze localized effects of genomic editing. It also is expected to identify neural connections at the whole brain level. In fact, last author Hiroki Ueda is optimistic about further application to even larger mammalian systems. “In the near future, we would like to apply CUBIC technology to whole-body imaging at single cell resolution.”

Yugiri from FFXIV ›

xantchawalker:

The more and more I look at her the less I feel that she is Dragon kin and the less I feel those thing on her heard are horns.

image

However, paying closer attention to her ears/horns in CS they can NOT horns because of the way they bounce and move while walking or while in the wind. Although…her…

instagram:

In the Artist’s Studio with @javicalleja

To see more of Javier’s work, follow @javicalleja on Instagram.

"I really hope I can make someone smile every day," says Spanish artist Javier Calleja (@javicalleja), who lives and works in Málaga. Javier’s bright, whimsical creations take the form of drawings, paintings and occasionally sculpture.

Since joining Instagram a year ago, Javier’s built a community around his playful, surreal aesthetic. “As an artist, Instagram gives me the opportunity to share my work around the world,” Javier explains. But Javier’s use of Instagram goes deeper, and he uses the platform to engage with his fans. “I’ll do art just for Instagram,” he says. “It’s a great testing ground.”

(dirtylittlestylewhoreeから)